Pyridostatin Trifluoroacetate Salt is a G-quadruplexe stabilizer with Kd of 490 nM in a cell-free assay, which targets a series of proto-oncogenes including c-kit, K-ras and Bcl-2.

G-quadruplexe (Cell-free assay):490 nM(Kd)

Pyridostatin通过激活DNA损伤检查点，降低MRC-5–SV40细胞和各种癌细胞系的增殖，并诱导细胞周期阻滞。同时，Pyridostatin通过与SRC中的G-四联体结构相互作用，减少MDA-MB-231细胞中依赖SRC的细胞移动性。此外，Pyridostatin还通过抑制G-四联体降低EBNA1的合成。

Cell lines: MRC-5–SV40 cells and various cancer cell linesConcentrations: 10 μMIncubation Time: 48 hMethod: Cells are plated at equal confluence and are left either untreated or were treated with 2 μM pyridostatin continually during the indicated time before harvesting the cells. Cells from individual plates are trypsinized and counted in a Coulter counter. Graphs represent the total cell numbers at each time interval, and the error bars represent the s.e.m. Data represent three independent experiments.